Family: Chuviridae
Genus: Doliuvirus
Distinguishing features
Doliuviruses are primarily distinguished from viruses of other genera in the family based on nucleic acid sequence and organization differences (Di Paola et al., 2022). Members of the genus Doliuvirus have L protein sequences that are >31% identical to each other.
Genome organization and replication
Doliuviruses have bisegmented linear genomes of about 11.5 kb (S1: about 6.8 kb; S2: about 4.7 kb) with four ORFs encoding a glycoprotein (GP) , a large (L) protein with an RNA-directed RNA polymerase (RdRP) domain, and proteins of unknown function. The replication cycle of doliuviruses remains to be elucidated.
Biology
The only classified doliuvirus, Mos8Chu0 chuvirus (MoCV), has been associated with dipteran insects, specifically mosquitoes (culicid Culiseta minnesotae Barr, 1957), in the USA (unpublished). Unclassified doliuviruses have been associated with mosquitoes in Brazil.
Species demarcation criteria
New doliuvirus species should be established when the amino-acid identities of their complete L protein sequence are <90% compared to other viruses in the genus (Di Paola et al., 2022).
Related, unclassified viruses
| Virus name | Accession number | Virus abbreviation | Reference |
| Croada virus | MF344588* | (Lara Pinto et al., 2017) | |
| Cumbaru virus | MF344596* | (Lara Pinto et al., 2017) | |
| Guato virus | KT966484* | (Pauvolid-Corrêa et al., 2016) | |
| Kaiowa virus | KT966481* | (Pauvolid-Corrêa et al., 2016) | |
| midge associated chuvirus M1C6 | LR701645* | (Modha et al., 2019) | |
| midge associated chuvirus M1C7 | LR701646* | (Modha et al., 2019) | |
| midge associated chuvirus M1C8 | LR701647* | (Modha et al., 2019) | |
| Uru chuvirus | MK780200* | (Maia et al., 2019) |
Virus names and virus abbreviations are not official ICTV designations.
* Incomplete genome