Subfamily: Alphairidovirinae

Genus: Lymphocystivirus


Distinguishing features

In contrast to the systemic infections seen with ranaviruses and megalocytiviruses, lymphocystivirus infections result in wart-like growths mainly on the skin of infected fish. These growths are composed of numerous, greatly enlarged, cells. Infected cells are commonly 100 μm in diameter and sometimes reach 1 mm. Lymphocystivirus-infected cells display a thick hyaline capsule, a central enlarged nucleus, and prominent basophilic DNA cytoplasmic inclusions. 



Particle size varies from 198 to 227 nm and may show a fringe of external fibrils. The presence of capsid fibers distinguishes lymphocystiviruses from members of the other two genera of vertebrate iridoviruses. 

Physicochemical and physical properties

Virions are heat labile. Infectivity is sensitive to treatment with ether or glycerol. 

Nucleic acid

Within the genus, genomes vary from 102,263 bp (lymphocystis disease virus 1, LCDV1) to 208,501bp (lymphocystis disease virus 3, LCDV3). A contour length measurement of 146 kbp for LCDV1 suggests a terminal redundancy of approximately 50%. Like other vertebrate iridovirids, the lymphocystivirus genome is highly methylated (22%) with 74% of cytosines within CpG dinucleotides methylated. The complete genomic sequence is known for LCDV1,         , and LCDV3, and partial sequences are known for several others. Surprisingly, the G+C content, but not the methylation status, of the three fully-sequenced lymphocystiviruses is more like that of invertebrate iridoviruses rather than other vertebrate iridoviruses (Table 2. Iridoviridae). Furthermore, the published genome of LCDV1 is much smaller than that of lymphocystis disease virus 2 (LCDV2) and LCDV3. Whether this reflects critical biological differences between LCDV1 and LCDV2/LCDV3, or whether it reflects a sequencing error, perhaps due to the presence of repeat regions, remains to be determined. 


Little is known about the protein composition of lymphocystiviruses. The reported genomes are sufficient to encode between 108–183 proteins. BLAST analysis of predicted ORFs suggests that many putative gene products may be unique to the genus. 

Genome organization and replication

The LCDV1 genome (103 kbp) contains 108 largely non-overlapping putative ORFs, 38 of which show significant homology to proteins of known function. These 38 ORFs represent 43% of the coding capacity of the genome. The presence of a DNA methyltransferase and a methyl-sensitive restriction endonuclease with specificity for a CCGG target site may be indicative of a restriction-modification system capable of degrading host genomic DNA while protecting viral DNA by specific methylation. LCDV1 DNA contains numerous short direct, inverted and palindromic repetitive sequence elements. The LCDV2 genome contains 178 non-overlapping ORFs, 103 of which are homologs to the corresponding ORFs of LCDV1 and 75 potential genes that were not found in LCDV1 or other iridovirids. Among these 75 genes, there are eight genes that contain conserved domains of cellular genes and 67 novel genes that do not show any significant homology with sequences in public databases. Although LCDV1 and LCDV2 possess 103 genes in common, their gene order is markedly different. Furthermore, a large number of tandem and overlapping repeated sequences are observed in the LCDV2 genome. 

The genome of LCDV3 contains 183 putative ORFs. BLASTp analysis found that 145 ORFs displayed significant similarity to other genes in the database. Of these 145, 129 best match those in LCDV2, 10 best match LCDV1, two match ORFs within scale drop disease virus (genus Megalocytivirus) and the remaining four best match either fish (3 ORFs) or spider (1 ORF) genes. 

A lack of cell lines suitable for in vitro replication has hindered analysis of LCDV biogenesis. Because few studies have been conducted, it has been assumed that LCDV replication is generally similar to frog virus 3. 


Lymphocystis disease was the first clinical illness linked with an iridovirus. However, although clinical manifestations of infection (i.e., wart-like or tumor-like growths on the skin of infected fish) were first noted near the end of the 19th century, the specific etiological agent was not identified until nearly 100 years later. 

LCDV1 and LCDV2 infect flounder and LCDV3 infects gilthead seabream. Isolates have been obtained from multiple species of fresh- and salt-water fish, but because sequence information is not available their taxonomic position is unclear. Infection targets fibroblasts and results in wart-like lesions comprising grossly hypertrophied cells occurring mostly in the skin and fins (Flugel 1985). The disease has been observed in over 100 teleost species although viral species other than Lymphocystis disease virus 1 may cause a similar clinical disease. The duration of infected cell growth and viral proliferation is highly variable (5 days to 9 months) and is likely to be temperature-dependent. Virions are released following degeneration of the lesions. Transmission is achieved by contact; external sites, including the gills, are the principal portals of entry. High host population densities and external trauma are believed to enhance transmission. Implantation and injection are also effective routes of transmission. The incidence of disease may be higher in the presence of certain fish ectoparasites. LCDV is not considered to be of major economic importance in fish since mortality is low. However, although infections are usually benign and self-limiting, there may be commercial concerns because the unsightly appearance of infected fish leads to market rejection. Moreover, mortalities may occur when infections involve the gills or when there is debilitation or secondary bacterial infection. 


The major capsid protein (MCP) is antigenic and protective antibodies are produced following exposure to this protein (de Groof et al., 2015). There are other immunogenic viral proteins including one of 60 kDa for which a polyclonal antiserum provides a diagnostic reagent (Cano et al., 2006). In addition, monoclonal antibodies have been raised against several lymphocystivirus proteins and used in indirect immunofluorescence (IFA), Western blot, and enzyme-linked immunosorbent (ELISA) assays (Cheng et al., 2006). 

Derivation of names

Isolates are named after the associated disease and the chronological order in which the sample was isolated (e.g., LCDV1), the geographic location of the isolate (e.g., LCDV-C, China), or the host from which it was isolated (e.g., LCDV-Sa, Sparus aurata,gilthead seabream). 

Species demarcation criteria 

LCDV1, LCDV2, and LCDV3 are distinguished from one another by differences in genome size, G+C content, genomic organization, and phylogenetic analysis. 

Phylogenetic analysis based on a concatenated set of 26 core iridovirid genes, or individual MCP, DNA polymerase and myristylated membrane protein genes, indicates that lymphocystivirus isolates display considerable sequence diversity. Overall the genome of LCDV3 shares 55% identity to LCDV2 and 39% with LCDV1 whereas LCDV1 and LCDV2 shared 42% identity to each other. Dot plot analysis of the three isolates showed little evidence of conserved gene order (Lopez-Bueno et al., 2016). Based on sequence differences among these isolates and the marked lack of genomic co-linearity, LCDV1, LCDV2, and LCDV3 represent three distinct species within the genus. Other lymphocystivirus isolates from yellow perch, rockfish, pearl gourami, glass fish and paradise fish have been reported and partial sequence data is available. In view of the above, the number of species within the genus is currently under review. 

Member species

The Member Species table enumerating important virus exemplars classified under each species of the genus is provided at the bottom of the page.


Related, unclassified viruses

Virus name

Accession numbera

Virus abbreviation

Genome (bp)



lymphocystitis disease virus-paradise fish






lymphocystis disease virus – painted glassfish






lymphocystis disease virus – pearl gourami






lymphocystis disease virus - rockfish






Virus names and abbreviations are not official ICTV designations. 

ND: not determined. 

a For the above isolates, the full genomic sequence has not yet been determined. Listed is the GenBank accession number for the major capsid protein gene.